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Nature | Electroporation can improve the efficienc
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3 posts
Oct 12, 2022
9:55 PM
Although no significant T cell toxicity was found in cells introduced with short strand oligodeoxynucleotides (ssODN) as a homologous recombination repair template, studies have shown that larger double-stranded DNA templates have significant cytotoxicity at high concentrations. However, in the experiment,personalised tailor tape, contrary to the expected results, the introduction of CRISPR-Cas9 ribosomal protein complex (RNP) and double-stranded DNA templates longer than 1 kb into human primary T cells by co-electroporation was less toxic than the introduction of double-stranded DNA templates alone. 2 Research process 1. First, RNP and a HDR repair template were electrotransfected into T cells, and the housekeeping gene RAB11a was knocked into the N fragment of GFP fusion gene. About 50% of CD4 + and CD8 + T cells expressed GFP, indicating that T cells could still maintain good proliferation ability and obtain a high efficiency of gene knockin. This result was confirmed by repeated experiments in T cells from different sources (fig. 1). Expand the full text Figure 1 2. The author's next step is to expand the scope and verify whether this gene editing technology is feasible at different locations in the whole genome. As a result,custom tailor tape, it was confirmed that gene knock-in could be performed using a non-viral vector method at different selected sites (fig. 2). This may occur at the site of naturally occurring or induced double-stranded DNA breaks in the cell (e.g., bespoken tape measure ,bra tape measure, off-site cleavage of RNP), Similar to the conclusion in the article, according to our experience, gene editing using "non-viral vector" method can achieve the advantages of "stability,custom tape measure, efficiency, safety and accuracy" through professional personalized program design. Teachers in need can contact us. Welcome to consult. Source: Marketing Center Jerry Original works of Jieyi, please indicate the source for reprinting Source: https://www.nature.com/articles/s41586-018-0326-5. · END · Return to Sohu to see more Responsible Editor:. tape-measure.com

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